Where can InSight be used?

1. Methodology
   • Translational assessment of a genetic engineering methodology to improve islet function for transplantation. van Krieken PP et al. EBioMedicine. 2019 Jul;45:529-541.
   • Local release of rapamycin by microparticles delays islet rejection within the anterior chamber of the eye. Fan Y et al. Sci Rep. 2019 Mar 8;9(1):3918.
   • The eye as a novel imaging site in diabetes research. Yang SN & Berggren PO. Pharmacol Ther. 2019 May;197:103-121
   • Pancreatic Islet Survival and Engraftment Is Promoted by Culture on Functionalized Spider Silk Matrices. Johansson U et al. PLoS One. 2015 Jun 19;10(6):e0130169.
   • Transplantation into the anterior chamber of the eye for longitudinal, non-invasive in vivo imaging with single-cell resolution in real-time. Abdulreda MH et al. J Vis Exp. 2013 Mar 10;(73):e50466.
   • Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye. Speier S at al. Nat Protoc. 2008;3(8):1278-86.
2. Study of islets of Langerhans
   • Alpha cell regulation of beta cell function. Moede T et al. Diabetologia. 2020 Oct;63(10):2064-2075.
   • In vivo Ca2+ dynamics in single pancreatic β cells. Jacob S et al. FASEB J. 2020 Jan;34(1):945-959.
   • Structural basis for delta cell paracrine regulation in pancreatic islets. Arrojo E Drigo R et al. Nat Commun. 2019 Aug 16;10(1):3700.
   • Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass. Paschen M et al. FASEB J. 2019 Jan;33(1):204-218.
   • Blocking Ca2+ Channel β3 Subunit Reverses Diabetes. Lee K et al. Cell Rep. 2018 Jul 24;24(4):922-934
   • Reporter islets in the eye reveal plasticity of the endocrine pancreas. Ilegems E et al. Proceedings of the National Academy of Sciece (PNAS) U S A. 2013 Nov 18
   • Paracrine Interactions within the Pancreatic Islet Determine the Glycemic Set Point. Rodriguez-Diaz R et al. Cell Metab. 2018 Mar 6;27(3):549-558.e4.
   • Kinetics of functional beta cell mass decay in a diphtheria toxin receptor mouse model of diabetes. van Krieken PP et al. Sci Rep. 2017 Sep 29;7(1):12440.
   • Pancreatic Islet Blood Flow Dynamics in Primates. Diez JA et al. Cell Rep. 2017 Aug 8;20(6):1490-1501.
   • Intraocular in vivo imaging of pancreatic islet cell physiology/pathology. Leibiger IB & Berggren P-O. Mol Metab. 2017 May 4;6(9):1002-1009.
   • The anterior chamber of the eye is a transplantation site that supports and enables visualisation of beta cell development in mice. Ali Y et al. Diabetologia. 2016 May;59(5):1007-11.
   • Liraglutide Compromises Pancreatic β Cell Function in a Humanized Mouse Model. Abdulreda MH et al. Cell Metab. 2016 Mar 8;23(3):541-6.
   • Non-invasive cell type selective in vivo monitoring of insulin resistance dynamics. Paschen M et al. Sci Rep. 2016 Feb 22;6:21448.
   • Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion. Ilegems E et al. Sci Rep. 2015 Jun 1;5:10740.
   • Apolipoprotein CIII links islet insulin resistance to β-cell failure in diabetes. Åvall K et al. Proc Natl Acad Sci U S A. 2015 May 19;112(20):E2611-9.
   • The pancreatic islet as a signaling hub. Barker CJ, Leibiger IB, Berggren PO. Advances in Biological Regulation. 2012 Sept 28.
   • Non-invasive in vivo model demonstrating the effects of autonomic innervation on pancreatic islet function. Rodriguez-Diaz R et al. Proceedings of the National Academy of Sciences (PNAS). Online Early Edition 10-14 December 2012
   • Non-invasive in vivo imaging of pancreatic β-cell function and survival – a perspective. Leibiger IB, Caicedo A, Berggren PO. Acta Physiol (Oxf), 2012 Feb;204(2):178-85
   • High-resolution, noninvasive longitudinal live imaging of immune responses. Ablulreda MH et al. Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12863-8.
   • Noninvasive in vivo imaging of pancreatic islet cell biology. S.Speier et.al. Technical reports, Nature Medicine, 2008 May;14(5):574-8
   • Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye. Speier S at al. Nat Protoc. 2008;3(8):1278-86.
3. Study of immune reactions during allorejection of the islets of Langerhans
   • Islet macrophages are associated with islet vascular remodeling and compensatory hyperinsulinemia during diabetes. Chittezhath M et al. Am J Physiol Endocrinol Metab. 2019 Dec 1;317(6):E1108-E1120.
   • In vivo imaging of type 1 diabetes immunopathology using eye-transplanted islets in NOD mice. Abdulreda MH et al. Diabetologia. 2019 Jul;62(7):1237-1250.High-resolution, noninvasive longitudinal live
   • Operational immune tolerance towards transplanted allogeneic pancreatic islets in mice and a non-human primate. Abdulreda MH et al. Diabetologia. 2019 May;62(5):811-821.
   • Intravital imaging of cytotoxic T lymphocytes. Faleo G et al. Methods Mol Biol. 2014;1186:121-9.
   • Imaging dynamics of CD11c⁺ cells and Foxp3⁺ cells in progressive autoimmune insulitis in the NOD mouse model of type 1 diabetes. Schmidt-Christensen A et al. Diabetologia. 2013 Dec;56(12):2669-78.
   • High-resolution, non-invasive longitudinal live imaging of immune responses. Midhat H. Albulreda et.al. PNAS, 2011 Aug 2;108(31)
4. Study of islet endothelial cells
   • Young capillary vessels rejuvenate aged pancreatic islets. Almaça J et al. Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):17612-7.
   • Donor Islet Endothelial Cells in Pancreatic Islet Revascularization. Nykvist D et.al. Diabetes, 2011 Oct;60(10):2571-7
5. Cancer
   • Noninvasive intravital high-resolution imaging of pancreatic neuroendocrine tumours. Balan M et al. Sci Rep. 2019 Oct 10;9(1):14636. 
6. Translation to the clinic
   • The anterior chamber of the eye as a clinical transplantation site for the treatment of diabetes: a study in a baboon model of diabetes. Perez VL et al. Diabetologia. 2011 May;54(5):1121-6.