Advantages of and protocols for using InSight Technology as an imaging platform are presented in the March 2013 issue of the Journal of Visualized Experiments.
Intravital, performed in the living organism, imaging has become an important tool in life sciences. A number of different technologies have emerged over the years and have been adapted to a variety of biological applications. Positron-emission tomography and magnetic resonance are examples of such technologies. None of them has though been able to combine such vital features as being non-invasive, longitudinal and providing single-cell resolution at the same time.
Researchers in Professor P-O Berggren’s group at both Karolinska Institutet and Diabetes Research Institute at the Miami Miller School of Medicine have over the past years excelled in InSight Technology. This intravital technique allows for longitudinal and non-invasive studies with single-cell resolution in several experimental animal models such as mice, rats and non-human primates.
The protocol of transplantation of islets of Langerhans into the anterior chamber of a mouse eye is published in the Journal of Visualized Experiments in March 2013.